## Aims
By the end of this practical you should feel confident doing the following:
- Fitting a detection function using `ds()`
- Checking detection functions using functions in package `Distance`
- Selecting models using AIC
- Estimating abundance (using R and maths!)
---
**Solutions are given between these horizontal lines with bold headers**
The "solutions" presented here are not definitive, many modelling options are possible. Our aim in this practical is to have some detection functions fitted to use later on and have you get familiar with the `Distance` package.
---
## Preamble
First need to load the requisite R libraries
```{r load-libraries, messages=FALSE}
library(Distance)
library(ggplot2)
library(knitr)
```
We can then load the data from the RData file (this needs to be in your [R working directory](https://support.rstudio.com/hc/en-us/articles/200711843-Working-Directories-and-Workspaces)):
```{r load-data}
load("spermwhale.RData")
```
## Exploring the data
Before setting off fitting detection functions, let's look at the relationship of various variables in the data.
The most important covariate in a distance sampling analysis is distance itself. We can plot a histogram of the distances to check that (1) we imported the data correctly and (2) it conforms to the usual shape for line transect data.
```{r eda-dist, fig.height=4, fig.cap="Distribution of observed perpendicular detection distances."}
hist(distdata$distance, xlab="Distance (m)",
main="Distance to sperm whale observations")
```
We can also see how many observations were recorded per Beaufort level ([some background on Beaufort](https://en.wikipedia.org/wiki/Beaufort_scale#Modern_scale) with photos). We can first use `table()` to see what values occur and how many there are:
```{r beaufort-table}
table(distdata$Beaufort)
```
The survey protocol on these surveys took an average of several observers' assessments of the weather, hence we get these decimal values. We can use the `breaks=` argument to `hist()` to bin these values to integer values. We'll plot the values from the segments at the same time, so we can get an idea of what the conditions were like over the whole survey, not just when we saw sperm whales:
```{r hist-beaufort, fig.cap="", fig.width=10}
par(mfrow=c(1,2))
hist(distdata$Beaufort, breaks=seq(0, 5, by=1),
main="Observations per Beaufort level",
xlab="Beaufort")
hist(segs$Beaufort, breaks=seq(0, 5, by=1),
main="Segments per Beaufort level",
xlab="Beaufort")
```
We can see here that most of the segments had a Beaufort >= 2 and very few had Beaufort of 0-1 (right plot). We can see then that there are few observations at the lowest Beaufort (left plot) because there just wasn't much time to observe things in those conditions. We see that with increasing Beaufort observations fall off (left plot), even as there were plenty of segments with those higher Beaufort levels (right plot).
We can create a rounded version of the variable using the `cut` function in R for later:
```{r beaufort-cut}
distdata$BeaufortCut <- cut(distdata$Beaufort, seq(0, 5, by=1),
include.lowest=TRUE,
ordered.result=TRUE)
```
Given we are including data from two different surveys we can also check that the surveys have similar histograms of distance (we'll check with the detection function below whether we need to include a covariate to control for this).
```{r hist-distance-survey, fig.cap="", fig.width=10}
par(mfrow=c(1,2))
hist(subset(distdata, Survey=="en04395")$distance,
main="", ylim=c(0, 30),
xlab="Distance (m)")
hist(subset(distdata, Survey=="GU0403")$distance,
main="", ylim=c(0, 30),
xlab="Distance (m)")
```
## Fitting detection functions
It's now time to fit some detection function models. We'll use the `ds()` function from the `Distance` package to fit the detection function. You can access the help file for the `ds()` function by typing `?ds` -- this will give you information about what the different arguments to the function are and what they do.
We can fit a very simple model with the following code:
```{r simple-df}
df_hn <- ds(data=distdata, truncation=6000, key="hn", adjustment=NULL)
```
Let's dissect the call and see what each argument means:
- `data=distdata`: the data to use to fit the model, as we prepared above.
- `truncation=6000`: set the truncation distance. Here, observations at distances greater than 6000m will be discarded before fitting the detection function.
- `key="hn"`: the key function to use for the detection function, in this case half-normal (`?ds` lists the other options).
- `adjustment=NULL`: adjustment term series to fit. `NULL` here means that no adjustments should be fitted (again `?ds` lists all options).
Other useful arguments for this practical are:
- `formula=`: gives the formula to use for the scale parameter. By default it takes the value `~1`, meaning the scale parameter is constant and not a function of covariates.
- `order=`: specifies the "order" of the adjustments to be used. This is a number (or vector of numbers) specifying the order of the terms. For example `order=2` fits order 2 adjustments, `order=c(2,3)` will fit a model with order 2 and 3 adjustments (mathematically, it only makes sense to include order 3 with order 2). By default the value is `NULL` which has `ds()` select the number of adjustments by AIC.
We set `truncation=6000` here, why? The truncation removes observations in the tail of the distribution. We do this because we only care about the detection function near 0, that has the most effect on $\hat{p}$ (since it's where the detection function is biggest). We need to trade-off between getting the shape right and not using lots of parameters modelling what's going on far from the observer and has little effect on the result. Here we use ~96% of the data. Len Thomas suggests $g(w)\approx 0.15$ (i.e., at the truncation distance the detection function should be about 0.15). You can play around with this if you're interested here by changing `truncation=` and seeing what the differences in $\hat{p}$ are (but push on to the next section if you're happy!).
---
**Trying to fit a few detection functions**
First we can try using a hazard-rate detection function:
```{r df-hr}
df_hr <- ds(data=distdata, truncation=6000, key="hr", adjustment=NULL)
```
We can also try out using covariates in the detection function. Sea state (as we discretized above), using a hazard-rate and half-normal key function:
```{r df-beau}
df_hn_beau <- ds(data=distdata, truncation=6000, key="hn",
adjustment=NULL, formula=~BeaufortCut)
df_hr_beau <- ds(data=distdata, truncation=6000, key="hr",
adjustment=NULL, formula=~BeaufortCut)
```
We can also include the observed group size:
```{r df-beau-size}
df_hn_beau_size <- ds(data=distdata, truncation=6000, key="hn",
adjustment=NULL, formula=~BeaufortCut+size)
df_hr_beau_size <- ds(data=distdata, truncation=6000, key="hr",
adjustment=NULL, formula=~BeaufortCut+size)
```
Again this is just a sampling of the possible models we could fit to the detectability data. Next we'll inspect these models...
---
### Summaries
We can look at the summary of the fitted detection function using the `summary()` function:
```{r simple-summary}
summary(df_hn)
```
---
**Looking at summaries**
I won't go through all of the above here, but just compare with and without covariates, let's compare `summary(df_hn)` output above to that for
```{r df_hr_beau_size-summary}
summary(df_hr_beau_size)
```
Note the difference in the `Average p` between the two models. In the covariate case, this is averaged over the detection probabilities for the different covariate values. We can look at the distribution of the probabilities of detection for each observation using the `p_dist_table` function:
```{r p-dist-table}
p_dist_table(df_hr_beau_size)
```
We can see there's a broad range of probabilities of detection estimated for this model that get averaged out in the above `summary` output. Always worth checking!
---
### Goodness of fit
Goodness of fit quantile-quantile plot and test results can be accessed using the `gof_ds()` function:
```{r simple-gof, fig.width=4, fig.height=4, fig.asp=1, fig.cap="Goodness of fit QQ plot of half-normal detection function."}
gof_ds(df_hn)
```
---
**Comparing Q-Q plots**
Let's run `gof_ds()` on each of our models and look at the corresponding plots arranged in a grid
```{r qq-compare, fig.width=5, fig.height=8}
# make a grid, 3 rows by 2 columns
par(mfrow=c(3,2))
gof_ds(df_hn, main="df_hn")
gof_ds(df_hr, main="df_hr")
gof_ds(df_hn_beau, main="df_hn_beau")
gof_ds(df_hr_beau, main="df_hr_beau")
gof_ds(df_hn_beau_size, main="df_hn_beau_size")
gof_ds(df_hr_beau_size, main="df_hr_beau_size")
```
We can see that overall the half-normal Q-Q plots are further away from the lines in each case than their corresponding hazard-rate models, which would be good grounds for prefering hazard-rate here. I'll not discard these models for now, just so I can show other properties.
It's hard to see what the difference is between the hazard-rate models at the moment, so we'll investigate those more below.
---
### Plotting
We can plot the models simply using the `plot()` function:
```{r simple-plot, fig.height=4, fig.cap="Half-normal detection function."}
plot(df_hn)
```
The dots on the plot indicate the distances where observations are. We can remove them (particularly useful for a model without covariates) using the additional argument `showpoints=FALSE` (try this out!).
---
**Plotting detection functions**
We'll start by plotting everthing in a big grid again, dropping points where there are not covariates in the model:
```{r plot-compare, fig.width=5, fig.height=8}
# make a grid, 3 rows by 2 columns
par(mfrow=c(3,2))
plot(df_hn, main="df_hn", showpoints=FALSE)
plot(df_hr, main="df_hr", showpoints=FALSE)
plot(df_hn_beau, main="df_hn_beau")
plot(df_hr_beau, main="df_hr_beau")
plot(df_hn_beau_size, main="df_hn_beau_size")
plot(df_hr_beau_size, main="df_hr_beau_size")
```
For the Beaufort case we can use the `add_df_covar_line` function to add lines to our plot, rather than using points:
```{r plot-lines, fig.width=5, fig.height=8}
# remember to remove the points
plot(df_hr_beau, main="df_hr_beau", showpoints=FALSE)
# make a data.frame with the BeaufortCut levels in it
bf_data <- data.frame(BeaufortCut=levels(df_hr_beau$ddf$data$BeaufortCut))
# draw the lines
add_df_covar_line(df_hr_beau, data=bf_data)
# draw a legend
legend(4300, 1, lty=1:6, legend=c("Average", bf_data$BeaufortCut))
```
the `data=` argument here is used to make a little `data.frame` with the levels of `BeaufortCut` in it. These plots can get a little more tricky with more than one covariate in the detection function.
---
## Now you try...
Now try fitting a few models and comparing them using AIC. Don't try to fit all possible models, just try a selection (say, a hazard-rate, a model with adjustments and a couple with different covariates). You can also try out changing the truncation distance.
Here's an example to work from. Some tips before you start:
* You can include as many models as you like in a given chunk (though you may find it more manageable to separate them up, remembering each time to give the chunk a unique name).
* You can run the current line of code in RStudio by hitting `Control+Enter` (on Windows/Linux; `Command+Enter` on Mac).
* Giving your models informative names will help later on! Here I'm using `df_` to indicate that this is a detection function, then shortened forms of the model form and covariates, separated by underscores, but use what makes sense to you (and future you!).
* It's best not to include covariates *and* adjustments in the same model. There is some disagreement about this, but from a practical perspective it's hard to make sure that your detection function is always decreasing (monotonic) at all levels of your covariate, so you can end up with models that don't make sense (so best to avoid that!).
Here's an example of adding covariates into the model using the `formula=` argument:
```{r trying-models}
df_hr_ss_size <- ds(distdata, truncation=6000, adjustment=NULL,
key="hr", formula=~Beaufort+size)
```
Once you have the hang of writing models and looking at the differences between them, you should move onto the next section.
## Model selection
Looking at the models individually can be a bit unwieldy -- it's nicer to put that data into a table and sort the table by the relevant statistic. The function `summarize_ds_models()` in the `Distance` package performs this task for us.
The code below will make a results table with relevant statistics for model selection in it. The `summarize_ds_models()` function takes a series of object names as its first argument. We can do that with the two models that I fitted like so:
```{r df-results}
model_table <- summarize_ds_models(df_hn, df_hr_ss_size, output="plain")
kable(model_table, digits=3, row.names = FALSE, escape=FALSE,
caption = "Comparison of half normal and hazard rate with sea state and group size.")
```
(You can add the models you fitted above into this list.)
---
**Model selection**
Let's expand that table with the models I fitted above...
```{r df-results2}
model_table <- summarize_ds_models(df_hn, df_hr, df_hn_beau, df_hr_beau, df_hn_beau_size, df_hr_beau_size, output="plain")
kable(model_table, digits=3, row.names = FALSE, escape=FALSE,
caption = "Comparison of half normal and hazard rate with sea state and group size.")
```
Looking at the table we see that the `df_hn_beau` model does best in AIC terms *but* when we look back to the Q-Q plots above, we can see that the fit is not the best there. It's best not to base our model selection on a single measure (say, AIC) and use multiple ways to assess our models. In this case the Q-Q plot is telling us that the half-normal models are not as good (though we can see from the table that the CramÃ©r-von Mises test is not significant at the 5% level for any of these models, hence the next comment).
---
#### A further note about model selection for the sperm whale data
Note that there is a considerable spike in our distance data. This may be down to observers guarding the trackline (spending too much time searching near zero distance). It's therefore possible that the hazard-rate model is overfitting to this peak. So we'd like to investigate results from the half-normal model too and see what the effects are on the final spatial models.
### Estimating abundance
Just for fun, let's estimate abundance from these models using a Horvtiz-Thompson-type estimator.
We know the Horvitz-Thompson estimator has the following form:
$$
\hat{N} = \frac{A}{a} \sum_{i=1}^n \frac{s_i}{p_i}
$$
we can calculate each part of this equation in R:
- `A` is the total area of the region we want to estimate abundance for. This was $A=5.285e+11 m^2$.
- `a` is the total area we surveyed. We know that the total transect length was 9,498,474m and the truncation distance. Knowing that $a=2wL$ we can calculate $a$.
- $s_i$ are the group sizes, they are stored in `df_hn$ddf$data$size`.
- $p_i$ are the probabilities of detection, we can obtain them using `predict(df_hn$ddf)$fitted`.
We know that in general operations are vectorised in R, so calculating `c(1, 2, 3)/c(4, 5, 6)` will give `c(1/4, 2/5, 3/6)` so we can just divide the results of getting the $s_i$ and $p_i$ values and then use the `sum()` function to sum them up.
Try out estimating abundance using the formula below using both `df_hn` and your favourite model from above:
---
**Calculating the Horvitz-Thompson estimate**
Let's try this out for our model `df_hr_beau`:
```{r ht-estimate}
# total area of the region we want to estimate abundance for
A <- 5.285e+11
# total area we surveyed (2wL)
a <- 2 * 6000 * 9498474
# group sizes
group_sizes <- df_hr_beau$ddf$data$size
# need to get those that are within truncation!
group_sizes <- group_sizes[df_hr_beau$ddf$data$distance <= 6000]
# probabilities of detection
probs <- predict(df_hr_beau)$fitted
# now the H-T estimator
Nhat_hr_beau <- (A/a) * sum(group_sizes/probs)
Nhat_hr_beau
```
So this is our "flat" estimate that we might want to bear in mind as we go through the exercises etc.
---
Note that in the solutions to this exercise (posted on the course website) I show how to use the function `dht()` to estimate abundance (and uncertainty) for a detection function analysis. This involves some more complex data manipulation steps, so we've left it out here in favour of getting to grips with the mathematics.
---
**Using `dht`**
We can do this analysis using the `dht` function in the `mrds` package too (see `?dht` for more information and the link at the end of the answer for more information). We can then also get an estimate of variance too. We need to setup some tables to do this...
The total area of the study area is $A=5.285e+11m^2$, we can use that to construct the region table, with one region in it:
```{r region-tab}
region.table <- data.frame(Region.Label = "StudyArea",
Area = 5.285e+11)
```
We need to put our segments back together into transects to assemble the sample table (you might need to re-[download the `spermwhale.RData` file](../data/spermwhale.RData) as I updated the data for this).
The code here is fiddly, so don't worry too much about understanding it. This is only because I'm trying to get `dsm` formatted data to work for `dht`! In practice this would be easier.
```{r sample-tab}
library(dplyr)
library(tidyr)
transects <- segs %>%
# include only the TransectID and Effort columns
select(TransectID, Effort) %>%
# by Transect...
group_by(TransectID) %>%
# add all the effort up
summarise(Effort = sum(Effort)) %>%
# Sample.Label is now the transect id
rename(Sample.Label = TransectID) %>%
# get only the unique rows
distinct()
# add the Region.Label, same for every row
transects$Region.Label <- "StudyArea"
```
We can look at this:
```{r check-sample-tab}
transects
```
We need to build a new observation table with the new `Sample.Label`s, so first create that data:
```{r distdata-table}
# we can use the obs table for the DSM and merge that
# with the segment data
obs_dht <- merge(obs, segs[,c("Sample.Label", "TransectID")],
by="Sample.Label")
# but use the transect IDs as Sample.Label
obs_dht$Sample.Label <- obs_dht$TransectID
# then just extract the columns you need
obs_dht <- obs_dht[, c("object", "Sample.Label")]
# and add the Region.Label
obs_dht$Region.Label <- "StudyArea"
```
Now we can use `dht` to calculate the abundance for `df_hr_beau`, say (here I'm just re-using the `dist_dht` table again as the observation data):
```{r Nhat-dht}
Nhat_dht_hr_beau <- dht(model=df_hr_beau$ddf,
region.table=region.table,
sample.table=transects,
obs.table=obs_dht)
```
We can then look at the abundance estimates. `dht` calculates this for individuals and at the group level ("clusters"):
```{r dht-results}
Nhat_dht_hr_beau
```
We can keep this abundance and CV in mind as we explore the spatial models through the course.
More examples of doing these kinds of analyses are available on the [distance sampling exercises page](http://examples.distancesampling.org).
---
#### Accounting for perception bias
It's common, especially in marine surveys, for animals at zero distance to be missed by observers. There are several ways to deal with this issue. For now, we are just going to use a very simply constant correction factor to inflate the abundance.
From Palka (2006), we think that observations on the track line were such that $g(0)=0.46$, we can apply that correction to our abundance estimate (in a very primitive way):
---
**Perception bias correction**
```{r ht-perception}
Nhat_hr_beau/0.46
```
---
This kind of correction works fine when it's appropriate to use a single number to adjust by, in general we'd like to model the perception bias, for example using "mark-recapture distance sampling" techniques.
### Save model objects
Save your top few models in an `RData` file, so we can load them up later on. We'll also save the distance data we used to fit out models.
---
**Saving model objects**
```{r save-models-01}
save(df_hn, df_hr, df_hn_beau, df_hr_beau, df_hn_beau_size,
df_hr_beau_size, df_hr_ss_size,
distdata,
file="df-models.RData")
```
---
You can check it worked by using the `load()` function to recover the models.
## References
Palka, D. L. (2006) *Summer Abundance Estimates of Cetaceans in US North Atlantic Navy Operating Areas*. Northeast Fisheries Science Center Reference Document 06-03